Anticorps Polyclonal de lapin anti-ACP5

• Phare
• Validé par KD/KO

ACP5 Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 11594-1-AP

Synonymes

ACP5, Tartrate resistant acid ATPase, TR AP, TRACP, TRAP, TrATPase, Type 5 acid phosphatase

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Galerie de données de validation

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  WB analysis using 11594-1-AP

  pig tonsil tissue was subjected to SDS PAGE followed by western blot with 11594-1-AP (ACP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 11594-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human ACP5 (11594-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: pig tonsil tissue,
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

11594-1-AP cible ACP5 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACP5 Protéine recombinante Ag2065
• Nom complet: acid phosphatase 5, tartrate resistant
• Masse moléculaire calculée: 325 aa, 37 kDa
• Numéro d’acquisition GenBank: BC025414
• Symbole du gène: ACP5
• Identification du gène (NCBI): 54
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACP5(acid phosphatase 5, tartrate resistant) is also named as TRAP, TrATPase and belongs to the metallophosphoesterase superfamily. It is an iron-containing glycoprotein with a molecular mass of approximately 34 kD. ACP5 is a histochemical marker of the osteoclast. It is also characteristic of monohistiocytes, particularly alveolar macrophages, and is associated with diverse pathological conditions, including hairy cell leukemia and AIDS encephalopathy.(PMID:10639488).

Publications

Species	Application	Title
human,mouse	IF	Nat Commun; Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.; Authors - Yinan Hu ; KO Validated
mouse		Redox Biol; GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux; Authors - Xiaoxiao Ji
mouse	IHC	Hypertension; Secreted Klotho Attenuates Inflammation-Associated Aortic Valve Fibrosis in Senescence-Accelerated Mice P1.; Authors - Jianglei Chen
mouse	IHC	Bioact Mater; Bisphosphonate-based hydrogel mediates biomimetic negative feedback regulation of osteoclastic activity to promote bone regeneration.; Authors - Zhuo Li
mouse		FASEB J; The function of the calcium channel Orai1 in osteoclast development.; Authors - Lisa J Robinson
human	IF	Front Oncol; Single-Cell RNA Sequencing Reveals the Migration of Osteoclasts in Giant Cell Tumor of Bone; Authors - Wenyu Feng