Anticorps Monoclonal anti-ACPP

ACPP Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G2C9

N° de cat : 60176-1-PBS

Synonymes

PAP, 1G2C9, 5'-NT, 5'-nucleotidase, Acid phosphatase 3

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Galerie de données de validation

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  WB analysis of K-562 using 60176-1-Ig (same clone as 60176-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 60176-1-Ig (same clone as 60176-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 60176-1-Ig(ACPP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 60176-1-Ig(ACPP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60176-1-PBS cible ACPP dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACPP Protéine recombinante Ag8578
• Nom complet: acid phosphatase, prostate
• Masse moléculaire calculée: 418 aa, 48 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC007460
• Symbole du gène: ACPP
• Identification du gène (NCBI): 55
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

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