Anticorps Polyclonal de lapin anti-ACRV1

ACRV1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 14040-1-AP

Synonymes

Acrosomal protein SP 10, acrosomal vesicle protein 1, ACRV1, D11S4365, SP 10, SPACA2

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Galerie de données de validation

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  WB analysis of DU 145 using 14040-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14040-1-AP (ACRV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14040-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14040-1-AP (ACRV1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 14040-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 14040-1-AP (ACRV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of DU 145 using 14040-1-AP

  IP Result of anti-ACRV1 (IP:14040-1-AP, 3ug; Detection:14040-1-AP 1:2000) with DU 145 cells lysate 560ug.

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  IHC staining of human testis using 14040-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14040-1-AP (ACRV1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 14040-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14040-1-AP (ACRV1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of PC-3 using 14040-1-AP

  Immunofluorescent analysis of PC-3 cells using 14040-1-AP (ACRV1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules LNCaP, cellules PC-3
• Résultats positifs en IP: cellules DU 145
• Résultats positifs en IHC: tissu testiculaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 10 publications below

Informations sur le produit

14040-1-AP cible ACRV1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACRV1 Protéine recombinante Ag5109
• Nom complet: acrosomal vesicle protein 1
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 13-36 kDa
• Numéro d’acquisition GenBank: BC014588
• Symbole du gène: ACRV1
• Identification du gène (NCBI): 56
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The protein ACRV1, also known as SP-10, is an acrosomal matrix protein, which is evolutionarily conserved among mammals. It is associated with the matrix of the acrosomal vesicle in developing spermatids and the acrosomal matrix and membranes of ejaculated sperm (PMID: 8288254). Human ACRV1 protein has 11 isoforms, and molecular weight ranges from 9 to 28 kDa. ACRV1 was predominantly located in round and elongated spermatids in the testis, indicating that ACRV1 may play an important role in mammalian spermatogenesis and may be a target of a contraceptive vaccine (PMID: 21488928; 8874713).

Publications

Species	Application	Title
mouse	IF	Nat Commun; TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice.; Authors - Deqiang Ding
mouse	IHC	Int J Mol Sci; BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis.; Authors - Chao Huang
rat	IF	Syst Biol Reprod Med; Recellularized rat testis scaffolds with embryoid bodies cells: a promising approach for tissue engineering; Authors - Vitória Frias Batista
mouse	IF	Bioinformatics; SATINN: an automated neural network-based classification of testicular sections allows for high-throughput histopathology of mouse mutants; Authors - Ran Yang
mouse	IF	Nat Commun; SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility; Authors - Jiali Liu
mouse	IF	Arch Toxicol; Extended exposure to tetrabromobisphenol A-bis(2,3-dibromopropyl ether) leads to subfertility in male mice at the late reproductive age; Authors - Yuan-Yuan Li