Anticorps Polyclonal de lapin anti-ACSF2

• Phare
• Validé par KD/KO

ACSF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 16140-1-AP

Synonymes

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2

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Galerie de données de validation

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  WB analysis of mouse lung using 16140-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16140-1-AP (ACSF2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human colon cancer using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16140-1-AP (ACSF2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16140-1-AP (ACSF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 16140-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 16140-1-AP (ACSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16140-1-AP cible ACSF2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACSF2 Protéine recombinante Ag9199
• Nom complet: acyl-CoA synthetase family member 2
• Masse moléculaire calculée: 615 aa, 68 kDa
• Poids moléculaire observé: 55-70 kDa
• Numéro d’acquisition GenBank: BC012053
• Symbole du gène: ACSF2
• Identification du gène (NCBI): 80221
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.

Publications

Species	Application	Title
rat	WB,IP	Prostaglandins Other Lipid Mediat; Targeted Lipidomics Analysis of Lysine 179 Acetylation of ACSF2 in Rat Hepatic Stellate Cells; Authors - Meili Wang
mouse,rat	WB,IHC	Life Sci; ACSF2-mediated ferroptosis is involved in ulcerative colitis; Authors - Lianxiang Luo
human	WB,IF	Free Radic Biol Med; Inhibition of ACSF2 protects against renal ischemia/reperfusion injury via mediating mitophagy in proximal tubular cells; Authors - Haoyu Shi ; KD Validated
human	IHC	Clin Cancer Res; Biomarker identification and risk prediction model development for differentiated thyroid carcinoma lung metastasis based on primary lesion proteomics; Authors - Xiaoqi Peng
human,mouse	WB,IHC,IF	Diabetologia; ACSF2 and lysine lactylation contribute to renal tubule injury in diabetes; Authors - Jingfang Chen
human	WB,CoIP	Mol Carcinog; M2 macrophage exosome-derived Apoc1 promotes ferroptosis resistance in osteosarcoma by inhibiting ACSF2 deubiquitination; Authors - Ping Yin

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