Anticorps Recombinant de lapin anti-ACSF2

ACSF2 Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241208C3

N° de cat : 84033-2-PBS

Synonymes

241208C3, ACSF 2, EC:6.2.1.2, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, UNQ493/PRO1009

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Galerie de données de validation

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  IHC staining of mouse colon using 84033-2-RR (same clone as 84033-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84033-2-RR (ACSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84033-2-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 84033-2-RR (same clone as 84033-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84033-2-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 84033-2-RR (same clone as 84033-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84033-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00952-1

  Cytometric bead array standard curve of MP00952-1, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-2-PBS. Detection antibody: 84033-3-PBS. Standard: Ag9199. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00952-2

  Cytometric bead array standard curve of MP00952-2, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-1-PBS. Detection antibody: 84033-2-PBS. Standard: Ag9199. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84033-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84033-2-PBS against Human ACSF2 was performed. The affinity constant is 0.221 nM.

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  Affinity and Kinetic Characterization of 84033-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84033-2-RR against Human ACSF2 were performed. The affinity constant is 0.595 nM.

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Informations sur le produit

84033-2-PBS cible ACSF2 dans les applications de IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACSF2 Protéine recombinante Ag9199
• Nom complet: acyl-CoA synthetase family member 2
• Masse moléculaire calculée: 615 aa, 68 kDa
• Numéro d’acquisition GenBank: BC012053
• Symbole du gène: ACSF2
• Identification du gène (NCBI): 80221
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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