Anticorps Recombinant de lapin anti-ACSF2

ACSF2 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241208D9

N° de cat : 84033-3-PBS

Synonymes

241208D9, ACSF 2, EC:6.2.1.2, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, UNQ493/PRO1009

---

Galerie de données de validation

• 

  WB analysis using 84033-3-RR (same clone as 84033-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84033-3-RR (ACSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84033-3-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 84033-3-RR (same clone as 84033-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug ACSF2 Recombinant antibody (84033-3-RR, Clone:241208D9) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84033-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00952-1

  Cytometric bead array standard curve of MP00952-1, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-2-PBS. Detection antibody: 84033-3-PBS. Standard: Ag9199. Range: 0.625-80 ng/mL.

• 

  Cytometric bead array standard curve of MP00952-3

  Cytometric bead array standard curve of MP00952-3, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-1-PBS. Detection antibody: 84033-3-PBS. Standard: Ag9199. Range: 2.5-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 84033-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84033-3-PBS against Human ACSF2 was performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 84033-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84033-3-RR against Human ACSF2 were performed. The affinity constant is 1.94 nM.

---

Informations sur le produit

84033-3-PBS cible ACSF2 dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACSF2 Protéine recombinante Ag9199
• Nom complet: acyl-CoA synthetase family member 2
• Masse moléculaire calculée: 615 aa, 68 kDa
• Poids moléculaire observé: 51-71 kDa
• Numéro d’acquisition GenBank: BC012053
• Symbole du gène: ACSF2
• Identification du gène (NCBI): 80221
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.

{{ptg:RelatedPrimaryAntibodies}}