Anticorps Polyclonal de lapin anti-ACSL1

• Phare
• Validé par KD/KO

ACSL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(62)

Avis (1)

N° de cat : 13989-1-AP

Synonymes

ACS1, Acyl CoA synthetase 1, Acyl-CoA synthetase 1, Arachidonate--CoA ligase, EC:6.2.1.15

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Galerie de données de validation

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  WB analysis using 13989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13989-1-AP (ACSL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 13989-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13989-1-AP (ACSL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 13989-1-AP

  IP result of anti-ACSL1 (IP:13989-1-AP, 4ug; Detection:13989-1-AP 1:1000) with mouse liver tissue lysate 3040 ug.

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  IHC staining of human lung squamous cell carcinoma using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 13989-1-AP (ACSL1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13989-1-AP (ACSL1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13989-1-AP (ACSL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13989-1-AP (ACSL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13989-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSL1 antibody (13989-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules L02, tissu de cervelet de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: human lung squamous cell carcinoma tissue, tissu cardiaque humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 54 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Informations sur le produit

13989-1-AP cible ACSL1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACSL1 Protéine recombinante Ag5059
• Nom complet: acyl-CoA synthetase long-chain family member 1
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 68 kDa, 78 kDa
• Numéro d’acquisition GenBank: BC050073
• Symbole du gène: ACSL1
• Identification du gène (NCBI): 2180
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACSL1(Long-chain-fatty-acid--CoA ligase 1) is also named as FACL1, FACL2, LACS, LACS1, LACS2 and belongs to the ATP-dependent AMP-binding enzyme family. ACSL1 is a 75 kDa protein that is associated peripherally with the plasma membrane(Brian M Wiczer, etc., 2006). ACSL1 is abundantly expressed in tissues, such as liver and brown fat, that metabolize substantial amounts of triglycerides as fuel, and as such, a deficiency in ACSL1 function could have a more profound affect in those cells, resulting in hepatosteatosis and potentially increased very low density lipoprotein production by the liver or decreased thermogenic capacity in brown adipose tissue(PMID:19429676). An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages (PMID:17259370). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
mouse	IF	Nat Cell Biol; Functional multi-organelle units control inflammatory lipid metabolism of macrophages; Authors - Julia A Zimmermann
human	WB	Hepatology; Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.; Authors - Ming-Da Wang
human	IF	Theranostics; Mesenchymal glioblastoma constitutes a major ceRNA signature in the TGF-β pathway.; Authors - Qixue Wang
human	WB, IHC	Cancer Res; Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway.; Authors - Ming Cui
mouse	WB	Oxid Med Cell Longev; Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.; Authors - Wei Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Thomas (Verified Customer) (10-23-2019)

Very good service, very good antibody

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: Rattus heart

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