Anticorps Polyclonal de lapin anti-ACSL3

• Phare
• Validé par KD/KO

ACSL3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20710-1-PBS

Synonymes

EC:6.2.1.3, EC:6.2.1.2, EC:6.2.1.15, Arachidonate--CoA ligase, ACSL 3

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 20710-1-AP (same clone as 20710-1-PBS)

  WB result of ACSL3 antibody (20710-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL3 transfected HEK-293 cells. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20710-1-AP (same clone as 20710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 20710-1-AP (same clone as 20710-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 20710-1-AP (same clone as 20710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20710-1-AP (ACSL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

20710-1-PBS cible ACSL3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: acyl-CoA synthetase long-chain family member 3
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: NM_004457
• Symbole du gène: ACSL3
• Identification du gène (NCBI): 2181
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.

{{ptg:RelatedPrimaryAntibodies}}