Anticorps Polyclonal de lapin anti-ACSL4/FACL4

• Phare
• Validé par KD/KO

ACSL4/FACL4 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(308)

Avis (1)

N° de cat : 22401-1-AP

Synonymes

ACSL4, FACL4, ACS4, ACSL 4, Arachidonate--CoA ligase

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Galerie de données de validation

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  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22401-1-AP

  WB result of ACSL4/FACL4 antibody (22401-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4/FACL4 transfected HeLa cells.

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  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22401-1-AP

  IP result of anti-ACSL4/FACL4 (IP:22401-1-AP, 4ug; Detection:22401-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.

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  IHC staining of mouse brain using 22401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, cellules RAW 264.7, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 272 publications below
• IHC: See 54 publications below
• IF: See 32 publications below
• IP: See 5 publications below
• CoIP: See 3 publications below

Informations sur le produit

22401-1-AP cible ACSL4/FACL4 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACSL4/FACL4 Protéine recombinante Ag18085
• Nom complet: acyl-CoA synthetase long-chain family member 4
• Masse moléculaire calculée: 711 aa, 79 kDa
• Poids moléculaire observé: 79 kDa, 74 kDa
• Numéro d’acquisition GenBank: BC034959
• Symbole du gène: ACSL4
• Identification du gène (NCBI): 2182
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

Publications

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
human	CoIP	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
rat	WB,IHC	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
human	WB	Adv Sci (Weinh); Sirtuin1 Suppresses Calcium Oxalate Nephropathy via Inhibition of Renal Proximal Tubular Cell Ferroptosis Through PGC-1α-mediated Transcriptional Coactivation; Authors - Chen Duan
human	WB	Adv Sci (Weinh); Development and Application of the CRISPR-dcas13d-eIF4G Translational Regulatory System to Inhibit Ferroptosis in Calcium Oxalate Crystal-Induced Kidney Injury; Authors - Ziqi He

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

YINGJIAN (Verified Customer) (06-24-2025)

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: H9C2 cells

ACSL4/FACL4 Antibody Western Blot validation (1:5000 dilution) in H9C2 cells (Cat no:22401-1-AP)

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