Anticorps Recombinant de lapin anti-ACSL4

• Phare
• Validé par KD/KO

ACSL4 Recombinant Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

3I9

N° de cat : 81196-1-RR

Synonymes

ACS4, ACSL4, FACL4, LACS 4, LACS4, MRX63, MRX68

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Galerie de données de validation

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  WB analysis using 81196-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81196-1-RR (ACSL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 81196-1-RR

  WB result of ACSL4 antibody (81196-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells.

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  IP experiment of HeLa using 81196-1-RR

  IP result of anti-ACSL4(IP:81196-1-RR, 4ug; Detection:81196-1-RR 1:3000) with HeLa cells lysate 1560 ug.

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  IHC staining of human stomach cancer using 81196-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 81196-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 81196-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 81196-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 81196-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 81196-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HSC-T6, cellules HT-29, cellules L02, cellules MG U-87, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral de rat, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

81196-1-RR cible ACSL4 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACSL4 Protéine recombinante Ag18085
• Nom complet: acyl-CoA synthetase long-chain family member 4
• Masse moléculaire calculée: 711 aa, 79 kDa
• Poids moléculaire observé: 70 kDa, 75 kDa
• Numéro d’acquisition GenBank: BC034959
• Symbole du gène: ACSL4
• Identification du gène (NCBI): 2182
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

Publications

Species	Application	Title
human,mouse	WB	Front Pharmacol; Epigallocatechin gallate alleviates high-fat diet-induced hepatic lipotoxicity by targeting mitochondrial ROS-mediated ferroptosis; Authors - Shi-Bin Ding
human	IHC,WB	MedComm (2020); Novel aspect of neprilysin in kidney fibrosis via ACSL4-mediated ferroptosis of tubular epithelial cells; Authors - Weijing Lai