Anticorps Monoclonal anti-ACSL4/FACL4

• Phare
• Validé par KD/KO

ACSL4/FACL4 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H5D3

N° de cat : 66617-1-PBS

Synonymes

ACSL4, FACL4, 1H5D3, ACS4, ACSL 4

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Galerie de données de validation

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  WB analysis using 66617-1-Ig (same clone as 66617-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66617-1-Ig (same clone as 66617-1-PBS)

  WB result of ACSL4 antibody (66617-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66617-1-Ig (same clone as 66617-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66617-1-Ig (same clone as 66617-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 66617-1-Ig (same clone as 66617-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66617-1-Ig (same clone as 66617-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66617-1-Ig (same clone as 66617-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 66617-1-Ig (same clone as 66617-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human ACSL4 (66617-1-Ig, Clone:1H5D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66617-1-PBS cible ACSL4/FACL4 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACSL4/FACL4 Protéine recombinante Ag18085
• Nom complet: acyl-CoA synthetase long-chain family member 4
• Masse moléculaire calculée: 711 aa, 79 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC034959
• Symbole du gène: ACSL4
• Identification du gène (NCBI): 2182
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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