Anticorps Recombinant de lapin anti-ACSL4/FACL4

• Phare
• Validé par KD/KO

ACSL4/FACL4 Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3I9

N° de cat : 81196-1-PBS

Synonymes

ACSL4, FACL4, EC:6.2.1.15, Arachidonate--CoA ligase, ACSL 4

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Galerie de données de validation

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  WB analysis using 81196-1-RR (same clone as 81196-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81196-1-RR (ACSL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 81196-1-RR (same clone as 81196-1-PBS)

  WB result of ACSL4 antibody (81196-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 81196-1-RR (same clone as 81196-1-PBS)

  IP result of anti-ACSL4/FACL4 (IP:81196-1-RR, 4ug; Detection:81196-1-RR 1:3000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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Informations sur le produit

81196-1-PBS cible ACSL4/FACL4 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACSL4/FACL4 Protéine recombinante Ag18085
• Nom complet: acyl-CoA synthetase long-chain family member 4
• Masse moléculaire calculée: 711 aa, 79 kDa
• Poids moléculaire observé: 70 kDa, 75 kDa
• Numéro d’acquisition GenBank: BC034959
• Symbole du gène: ACSL4
• Identification du gène (NCBI): 2182
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

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