Anticorps Monoclonal anti-ACSM5

ACSM5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2D5D6

N° de cat : 67334-1-Ig

Synonymes

2D5D6, ACSM 5, Acyl-CoA synthetase medium-chain family member 5, Acyl-coenzyme A synthetase ACSM5, mitochondrial, EC:6.2.1.2

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Galerie de données de validation

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  WB analysis of 4T1 using 67334-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 67334-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67334-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-231 using 67334-1-Ig

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67334-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67334-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67334-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67334-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67334-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM5 antibody (67334-1-Ig, Clone: 2D5D6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules 4T1, cellules MDA-MB-231, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67334-1-Ig cible ACSM5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACSM5 Protéine recombinante Ag10056
• Nom complet: acyl-CoA synthetase medium-chain family member 5
• Masse moléculaire calculée: 208 aa, 23 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC016703
• Symbole du gène: ACSM5
• Identification du gène (NCBI): 54988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Arthritis Res Ther; Integrative analysis of genome-wide DNA methylation and single-nucleotide polymorphism identified ACSM5 as a suppressor of lumbar ligamentum flavum hypertrophy.; Authors - Yanlin Cao

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