Anticorps Monoclonal anti-ACSM5

ACSM5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D5D6

N° de cat : 67334-1-PBS

Synonymes

2D5D6, ACSM 5, Acyl-CoA synthetase medium-chain family member 5, Acyl-coenzyme A synthetase ACSM5, mitochondrial, EC:6.2.1.2

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Galerie de données de validation

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  WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67334-1-Ig (same clone as 67334-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-231 using 67334-1-Ig (same clone as 67334-1-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67334-1-Ig (same clone as 67334-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM5 antibody (67334-1-Ig, Clone: 2D5D6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67334-1-PBS cible ACSM5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACSM5 Protéine recombinante Ag10056
• Nom complet: acyl-CoA synthetase medium-chain family member 5
• Masse moléculaire calculée: 208 aa, 23 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC016703
• Symbole du gène: ACSM5
• Identification du gène (NCBI): 54988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

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