Anticorps Polyclonal de lapin anti-ACSS2

• Phare
• Validé par KD/KO

ACSS2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(22)

Avis (1)

N° de cat : 16087-1-AP

Synonymes

ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1

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Galerie de données de validation

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  WB analysis using 16087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16087-1-AP

  IP result of anti-ACSS2 (IP:16087-1-AP, 4ug; Detection:16087-1-AP 1:500) with HepG2 cells lysate 5600 ug.

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  IHC staining of human brain using 16087-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 16087-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 synthetase antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules Neuro-2a, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

16087-1-AP cible ACSS2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACSS2 Protéine recombinante Ag9121
• Nom complet: acyl-CoA synthetase short-chain family member 2
• Masse moléculaire calculée: 701 aa, 79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC010141
• Symbole du gène: ACSS2
• Identification du gène (NCBI): 55902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). ACSS2 has two isoforms with the molecular weight of 78 and 80 kDa.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
human,mouse	WB,IHC,IF	Nat Commun; ACSS2 protects against alcohol-induced hepatocyte ferroptosis through regulation of hepcidin expression; Authors - Mengyao Wang
mouse	WB	Mol Cell; Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.; Authors - Anyuan He
	WB	Plant Physiol; FDX5 deletion affects metabolism of algae during the different phases of S-deprivation.; Authors - Venkataramanan Subramanian
mouse	WB	Antioxidants (Basel); Bisdemethoxycurcumin Alleviates Dextran Sodium Sulfate-Induced Colitis via Inhibiting NLRP3 Inflammasome Activation and Modulating the Gut Microbiota in Mice; Authors - Jingfei Zhang
mouse	WB,IF	Am J Physiol Gastrointest Liver Physiol; N-glycosylation of SCAP exacerbates hepatocellular inflammation and lipid accumulation via ACSS2-mediated histone H3K27 acetylation; Authors - Xuemei Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kishor (Verified Customer) (01-30-2019)

Good results in western blotting at 1:1000 dilution for human cell lines and rat liver tissue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue

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