Anticorps Polyclonal de lapin anti-ACSS2

• Phare
• Validé par KD/KO

ACSS2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16087-1-PBS

Synonymes

ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1

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Galerie de données de validation

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  WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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  WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 16087-1-AP (same clone as 16087-1-PBS)

  IP result of anti-ACSS2 (IP:16087-1-AP, 4ug; Detection:16087-1-AP 1:500) with HepG2 cells lysate 5600 ug. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 synthetase antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16087-1-PBS cible ACSS2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACSS2 Protéine recombinante Ag9121
• Nom complet: acyl-CoA synthetase short-chain family member 2
• Masse moléculaire calculée: 701 aa, 79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC010141
• Symbole du gène: ACSS2
• Identification du gène (NCBI): 55902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). ACSS2 has two isoforms with the molecular weight of 78 and 80 kDa.

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