Anticorps Polyclonal de lapin anti-ACTA1-Specific

ACTA1-Specific Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17521-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hysteromyoma using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hysteromyoma using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  FC experiment of C2C12 using 17521-1-AP (same clone as 17521-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTA1-Specific (17521-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17521-1-PBS cible ACTA1-Specific dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: actin, alpha 1, skeletal muscle
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 39-42 kDa
• Numéro d’acquisition GenBank: NM_001100
• Symbole du gène: ACTA1
• Identification du gène (NCBI): 58
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.

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