Anticorps Recombinant de lapin anti-ACTA1-Specific

ACTA1-Specific Recombinant Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, poulet, rat, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

5B15

N° de cat : 82714-5-RR

Synonymes

ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle

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Galerie de données de validation

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  WB analysis using 82714-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82714-5-RR (ACTA1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse skeletal muscle using 82714-5-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of mouse skeletal muscle using 82714-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 82714-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique de porc, tissu de muscle squelettique de rat
• Résultats positifs en IF-P: tissu de muscle squelettique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82714-5-RR cible ACTA1-Specific dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, poulet, rat, souris

• Réactivité: Humain, porc, poulet, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: actin, alpha 1, skeletal muscle
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 39-42 kDa
• Numéro d’acquisition GenBank: NM_001100
• Symbole du gène: ACTA1
• Identification du gène (NCBI): 58
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.

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