Anticorps Polyclonal de lapin anti-beta actin

beta actin Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10230-1-AP

Synonymes

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

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Galerie de données de validation

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Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below

Informations sur le produit

10230-1-AP cible beta actin dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: bovin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: beta actin Protéine recombinante Ag0297
• Nom complet: actin, beta
• Masse moléculaire calculée: 375 aa, 42 kDa
• Numéro d’acquisition GenBank: BC002409
• Symbole du gène: ACTB
• Identification du gène (NCBI): 60
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Beta Actin has been widely used as the internal control in bioscience. At least six isoforms are known in mammals. ALPHA-actin was predominant in single cells and BETA-actin was major in the cultured cells. Nonmuscle BETA- and GAMMA-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells. ALPHA-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, ALPHA- and GAMMA-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. This antibody can detect endogenous level of β-Actin and may cross-react with other isoforms of actin family.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.

Publications

Species	Application	Title
human	WB	Int J Nanomedicine; Integration of Dual Targeting and Dual Therapeutic Modules Endows Self-Assembled Nanoparticles with Anti-Tumor Growth and Metastasis Functions.; Authors - Biao Chen
human	WB	J Cell Biochem; Dependence of artesunate on long noncoding RNA-RP11 to inhibit epithelial-mesenchymal transition of hepatocellular carcinoma.; Authors - Wu Jing
bovine	WB	Biol Trace Elem Res; The Protective Effect of Selenium on Bovine Mammary Epithelial Cell Injury Caused by Depression of Thioredoxin Reductase.; Authors - Yongmei Guo
human	WB	BMC Urol; Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway.; Authors - Cai Lv
	WB	Int J Mol Med; miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells.; Authors - Tao Han
human	WB	Int J Biol Macromol; Scavenger receptor a mediates glycated LDL transcytosis across endothelial cells to promote atherosclerosis; Authors - Wenzhuo Cheng