Anticorps Polyclonal de lapin anti-Alpha cardiac muscle actin

Alpha cardiac muscle actin Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 11032-1-AP

Synonymes

ACTC1, Alpha actin, ACTC, ACTC 1, ACTC1 cardiac muscle 1

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Galerie de données de validation

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  WB analysis of mouse heart using 11032-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11032-1-AP (alpha Actin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11032-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11032-1-AP (alpha Actin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11032-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11032-1-AP (alpha Actin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11032-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11032-1-AP (alpha Actin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 11032-1-AP

  IP result of anti-Alpha Actin (IP:11032-1-AP, 4ug; Detection:11032-1-AP 1:800) with mouse heart tissue lysate 3200ug.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu de thymus de souris
• Résultats positifs en IP: tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

11032-1-AP cible Alpha cardiac muscle actin dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha cardiac muscle actin Protéine recombinante Ag1471
• Nom complet: actin, alpha, cardiac muscle 1
• Masse moléculaire calculée: 377 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC009978
• Symbole du gène: ACTC1
• Identification du gène (NCBI): 70
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	FASEB J; Zbtb20 deficiency causes cardiac contractile dysfunction in mice.; Authors - An-Jing Ren
human	WB	Int J Biol Sci; MnSOD Lysine 68 acetylation leads to cisplatin and doxorubicin resistance due to aberrant mitochondrial metabolism.; Authors - Yucheng Gao
human	IHC	Stem Cells Dev; A model of early human embryonic stem cell differentiation reveals inter- and intracellular changes on transition to squamous epithelium.; Authors - Galat Vasiliy V
human	WB	Onco Targets Ther; Clinical Significance and Oncogenic Activity of GRWD1 Overexpression in the Development of Colon Carcinoma.; Authors - Xin Zhou

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