Anticorps Monoclonal anti-ACTN2

ACTN2 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D5F9

N° de cat : 68223-1-PBS

Synonymes

actinin, alpha 2, ACTN2, Alpha actinin 2, CMD1AA, F actin cross linking protein

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Galerie de données de validation

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  WB analysis using 68223-1-Ig (same clone as 68223-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68223-1-Ig (ACTN2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68223-1-PBS in a different storage buffer formulation.

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  WB analysis of rat colon using 68223-1-Ig (same clone as 68223-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68223-1-Ig (ACTN2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68223-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68223-1-PBS cible ACTN2 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACTN2 Protéine recombinante Ag5578
• Nom complet: actinin, alpha 2
• Masse moléculaire calculée: 104 kDa
• Poids moléculaire observé: 103 kDa
• Numéro d’acquisition GenBank: BC051770
• Symbole du gène: ACTN2
• Identification du gène (NCBI): 88
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).

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