Anticorps Recombinant de lapin anti-ACTN2

ACTN2 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250229F6

N° de cat : 85857-4-PBS

Synonymes

Actinin alpha 2, actinin, alpha 2, ACTN 2, Alpha actinin 2, Alpha-actinin skeletal muscle isoform 2

---

Galerie de données de validation

• 

  WB analysis using 85857-4-RR (same clone as 85857-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85857-4-RR (ACTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 85857-4-RR (same clone as 85857-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85857-4-RR (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 85857-4-RR (same clone as 85857-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 85857-4-RR (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

• 

  IF Staining of H9C2 using 85857-4-RR (same clone as 85857-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ACTN2 antibody (85857-4-RR, Clone: 250229F6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP02153-1

  Cytometric bead array standard curve of MP02153-1, ACTN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85857-4-PBS. Detection antibody: 85857-3-PBS. Standard: Ag5459. Range: 0.781-100 ng/mL.

---

Informations sur le produit

85857-4-PBS cible ACTN2 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACTN2 Protéine recombinante Ag5459
• Nom complet: actinin, alpha 2
• Masse moléculaire calculée: 104 kDa
• Poids moléculaire observé: 103 kDa
• Numéro d’acquisition GenBank: BC051770
• Symbole du gène: ACTN2
• Identification du gène (NCBI): 88
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles, and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).

{{ptg:RelatedPrimaryAntibodies}}