Anticorps Recombinant de lapin anti-ACTN3

ACTN3 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241168A5

N° de cat : 84356-1-PBS

Synonymes

Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3, actinin, alpha 3

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Galerie de données de validation

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  WB analysis of rat skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84356-1-RR (ACTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84356-1-RR (ACTN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (84356-1-RR, Clone: 241168A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84356-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84356-1-RR against Human ACTN3 were performed. The affinity constant is 0.116 nM.

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Informations sur le produit

84356-1-PBS cible ACTN3 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACTN3 Protéine recombinante Ag36709
• Nom complet: actinin, alpha 3
• Masse moléculaire calculée: 901 aa, 103 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC099647
• Symbole du gène: ACTN3
• Identification du gène (NCBI): 89
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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