Anticorps Polyclonal de lapin anti-ACTN4

• Phare
• Validé par KD/KO

ACTN4 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 19096-1-AP

Synonymes

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

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Galerie de données de validation

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  WB analysis using 19096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19096-1-AP (ACTN4 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19096-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19096-1-AP (ACTN4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19096-1-AP

  IP Result of anti-ACTN4 (IP:19096-1-AP, 5ug; Detection:19096-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human lung cancer using 19096-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19096-1-AP (ACTN4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 19096-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19096-1-AP (ACTN4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19096-1-AP (ACTN4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19096-1-AP (ACTN4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19096-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 19096-1-AP (ACTN4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 19096-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 19096-1-AP (ACTN4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 19096-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ACTN4 antibody 19096-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of MCF-7 using 19096-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 19096-1-AP (ACTN4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 19096-1-AP

  Immunofluorescent analysis of Hela cells, using ACTN4 antibody 19096-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HeLa using 19096-1-AP

  1X10^6 HeLa cells were stained with 0.2ug ACTN4 antibody (19096-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules C2C12, cellules HeLa, cellules Jurkat, cellules NIH/3T3, muscle squelettique de rat, muscle squelettique de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

19096-1-AP cible ACTN4 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACTN4 Protéine recombinante Ag13546
• Nom complet: actinin, alpha 4
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 100-105 kDa
• Numéro d’acquisition GenBank: BC005033
• Symbole du gène: ACTN4
• Identification du gène (NCBI): 81
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 100-105 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

Publications

Species	Application	Title
human	ELISA	J Thorac Oncol; Alpha-actinin 4 is Associated with Cancer Cell Motility and is a Potential Biomarker in Non-Small-Cell Lung Cancer.; Authors - Ming-Chuan Wang
human	WB	Oncogene; MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.; Authors - Agarwal N N
human	WB	Cancer Immunol Immunother; Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.; Authors - Xiuxing Chen
rat	WB,IF	Sci Rep; Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.; Authors - Hongfei Ge
mouse	IF	J Cell Sci; GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region; Authors - Paroma Chatterjee
human	WB	Amino Acids; Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.; Authors - Chen Bai

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