Anticorps Monoclonal anti-ACTN4

• Phare
• Validé par KD/KO

ACTN4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E12B8

N° de cat : 66628-1-PBS

Synonymes

Alpha actinin 4, 1E12B8, ACTININ 4, Actinin alpha 4, actinin, alpha 4

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Galerie de données de validation

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  WB analysis using 66628-1-Ig (same clone as 66628-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66628-1-Ig (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

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  WB analysis using 66628-1-Ig (same clone as 66628-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66628-1-Ig (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66628-1-Ig (same clone as 66628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66628-1-Ig (same clone as 66628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66628-1-Ig (same clone as 66628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66628-1-Ig (same clone as 66628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66628-1-PBS cible ACTN4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACTN4 Protéine recombinante Ag13549
• Nom complet: actinin, alpha 4
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 105 and 80 kDa
• Numéro d’acquisition GenBank: BC005033
• Symbole du gène: ACTN4
• Identification du gène (NCBI): 81
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

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