Anticorps Monoclonal anti-ACTR3B

ACTR3B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G12C6

N° de cat : 68037-1-PBS

Synonymes

1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B

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Galerie de données de validation

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  WB analysis using 68037-1-Ig (same clone as 68037-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68037-1-PBS in a different storage buffer formulation.

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  WB analysis using 68037-1-Ig (same clone as 68037-1-PBS)

  Brain tissues were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68037-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68037-1-Ig (same clone as 68037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68037-1-Ig (ACTR3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68037-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68037-1-Ig (same clone as 68037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68037-1-Ig (ACTR3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68037-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68037-1-Ig (same clone as 68037-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ACTR3B antibody (68037-1-Ig, Clone: 1G12C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68037-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68037-1-PBS cible ACTR3B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACTR3B Protéine recombinante Ag10218
• Nom complet: ARP3 actin-related protein 3 homolog B (yeast)
• Masse moléculaire calculée: 418 aa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC008682
• Symbole du gène: ACTR3B
• Identification du gène (NCBI): 57180
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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