Anticorps Polyclonal de lapin anti-Arp5/ACTR5

• Phare
• Validé par KD/KO

Arp5/ACTR5 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 21505-1-AP

Synonymes

Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, hARP5, Sarcoma antigen NY SAR 16

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Galerie de données de validation

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  WB analysis of mouse cerebellum using 21505-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21505-1-AP

  WB result of Arp5/ACTR5 antibody (21505-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells.

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  WB analysis of mouse heart using 21505-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 21505-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21505-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 21505-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21505-1-AP

  IP Result of anti-Arp5/ACTR5 (IP:21505-1-AP, 4ug; Detection:21505-1-AP 1:500) with HEK-293 cells lysate 1120ug.

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  IF Staining of A549 using 21505-1-AP

  Immunofluorescent analysis of A549 cells using 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21505-1-AP

  Immunofluorescent analysis of HepG2 cells, using ACTR5 antibody 21505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, cellules HEK-293, tissu cardiaque de souris, tissu de muscle squelettique humain, tissu ovarien de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IF: cellules A549, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

21505-1-AP cible Arp5/ACTR5 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Arp5/ACTR5 Protéine recombinante Ag15976
• Nom complet: ARP5 actin-related protein 5 homolog (yeast)
• Masse moléculaire calculée: 607 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC038402
• Symbole du gène: ACTR5
• Identification du gène (NCBI): 79913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)

Publications

Species	Application	Title
mouse	WB	EMBO J; LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.; Authors - Yanying Wang
rat	WB,IF	J Cell Biol; Arp5 is a key regulator of myocardin in smooth muscle cells.; Authors - Tsuyoshi Morita ; KD Validated
mouse	WB,IF,IP	Elife; Actin-related protein 5 functions as a novel modulator of MyoD and MyoG in skeletal muscle and in rhabdomyosarcoma.; Authors - Tsuyoshi Morita ; KD Validated
human	WB	Cell Rep; Activation of the CARD8 Inflammasome Requires a Disordered Region.; Authors - Ashley J Chui