Anticorps Recombinant de lapin anti-ACVR1B

ACVR1B Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230260C1

N° de cat : 83026-6-PBS

Synonymes

Activin receptor-like kinase 4, Activin receptor type-1B, Activin receptor type 1B, Activin receptor like kinase 4, activin A receptor, type IB

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Galerie de données de validation

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  WB analysis using 83026-6-RR (same clone as 83026-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83026-6-RR (ACVR1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 83026-6-RR (same clone as 83026-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83026-6-RR (ACVR1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83026-6-RR (same clone as 83026-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83026-6-RR (ACVR1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83026-6-RR (same clone as 83026-6-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug ACVR1B Recombinant antibody (83026-6-RR, Clone:230260C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83026-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83026-6-RR against Human ACVR1B were performed. The affinity constant is 35.6 pM.

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Informations sur le produit

83026-6-PBS cible ACVR1B dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACVR1B Protéine recombinante Ag0129
• Nom complet: activin A receptor, type IB
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC000254
• Symbole du gène: ACVR1B
• Identification du gène (NCBI): 91
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACVR1B (ALK4, activin A receptor type 1B) is a transforming growth factor-beta (TGF-beta) superfamily member (PMID: 37020544). A potential role for ACVR1B in regulating muscle mass is that it is part of the transforming growth factor b (TGFb) pathway regulating myostatin (PMID: 21063444).

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