Anticorps Polyclonal de lapin anti-ACVRL1

ACVRL1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14745-1-PBS

Synonymes

ALK-1, ALK1, ALK 1, ACVRLK1, Activin receptor-like kinase 1

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Galerie de données de validation

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  WB analysis of human placenta using 14745-1-AP (same clone as 14745-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 14745-1-AP (same clone as 14745-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 14745-1-AP (same clone as 14745-1-PBS)

  IP result of anti-ACVRL1 (IP:14745-1-AP, 4ug; Detection:14745-1-AP 1:300) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 14745-1-AP (same clone as 14745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14745-1-AP (ACVRL1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 14745-1-AP (same clone as 14745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 14745-1-AP (same clone as 14745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 14745-1-AP (same clone as 14745-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug ACVRL1 Polyclonal antibody (14745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14745-1-PBS cible ACVRL1 dans les applications de WB, IHC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACVRL1 Protéine recombinante Ag6449
• Nom complet: activin A receptor type II-like 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC042637
• Symbole du gène: ACVRL1
• Identification du gène (NCBI): 94
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.

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