Anticorps Polyclonal de lapin anti-ADA

ADA Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (2)

N° de cat : 13328-1-AP

Synonymes

EC:3.5.4.4, ADA1

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Galerie de données de validation

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  WB analysis of HeLa using 13328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13328-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 13328-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse small intestine using 13328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 13328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules Jurkat, cellules Sp2/0
• Résultats positifs en IHC: tissu d'estomac humain, tissu d'amygdalite humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'intestin grêle de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 3 publications below

Informations sur le produit

13328-1-AP cible ADA dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADA Protéine recombinante Ag4128
• Nom complet: adenosine deaminase
• Masse moléculaire calculée: 363 aa, 41 kDa
• Poids moléculaire observé: 45-47 kDa
• Numéro d’acquisition GenBank: BC040226
• Symbole du gène: ADA
• Identification du gène (NCBI): 100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adenosine deaminase (ADA, EC 3.5.4.4), which has been found in plants, bacteria, and mammals including humans and others, catalyzes the hydrolysis of adenosine to yield inosine. It was demonstrated that ADA also catalyzes the hydrolysis of the derivatives of nucleoside adenosine such as 2′-deoxyadenosine. ADA is expressed on the surface of T and B cells and plays an important role in the development of immune system. (PMID: 26038697)

Publications

Species	Application	Title
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
mouse	WB	Eur J Pharmacol; Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.; Authors - Gerald Wölkart
mouse	WB,IHC	Cell Death Dis; Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation; Authors - Mei-Xin Li
human	WB	bioRxiv; The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma; Authors - Ying-Jiun C Chen
	WB	NPJ Biofilms Microbiomes; Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model; Authors - Yang Fu
mouse	WB	Animal Model Exp Med; The cumulative analgesic effect of repeated electroacupuncture is modulated by Adora3 in the SCDH of mice with neuropathic pain; Authors - Faisal Ayub Kiani

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Neta (Verified Customer) (07-28-2022)

antibody worked, signal slightly different than reported but overall OK.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse small intestine

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with NaCit antigen retrieval ph=6.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (neurons) at 3dpf

ADA Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (neurons) at 3dpf (Cat no:13328-1-AP)

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