Anticorps Monoclonal anti-ADA

• Phare
• Validé par KD/KO

ADA Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

2C4E9

N° de cat : 67870-1-Ig

Synonymes

ADA, Adenosine aminohydrolase, adenosine deaminase

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Galerie de données de validation

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  WB analysis using 67870-1-Ig

  Jurkat and MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67870-1-Ig

  WB result of ADA antibody (67870-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADA transfected Jurkat cells.

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  WB analysis of LNCaP using 67870-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67870-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 67870-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit spleen using 67870-1-Ig

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67870-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of U2OS using 67870-1-Ig

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ADA (67870-1-Ig, Clone:2C4E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules LNCaP, cellules MOLT-4, cellules Sp2/0, tissu splénique de lapin, tissu splénique de porc, tissu splénique de rat
• Résultats positifs en cytométrie: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67870-1-Ig cible ADA dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADA Protéine recombinante Ag31326
• Nom complet: adenosine deaminase
• Masse moléculaire calculée: 363 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC040226
• Symbole du gène: ADA
• Identification du gène (NCBI): 100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.