Anticorps Monoclonal anti-ADA

• Phare
• Validé par KD/KO

ADA Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

2C4E9

N° de cat : 67870-1-PBS

Synonymes

2C4E9, ADA1, EC:3.5.4.4

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Galerie de données de validation

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  WB analysis using 67870-1-Ig (same clone as 67870-1-PBS)

  Jurkat and MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67870-1-Ig (same clone as 67870-1-PBS)

  WB result of ADA antibody (67870-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADA transfected Jurkat cells. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67870-1-Ig (same clone as 67870-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67870-1-Ig (same clone as 67870-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 67870-1-Ig (same clone as 67870-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit spleen using 67870-1-Ig (same clone as 67870-1-PBS)

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67870-1-Ig (same clone as 67870-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 67870-1-Ig (same clone as 67870-1-PBS)

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ADA (67870-1-Ig, Clone:2C4E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 67870-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 67870-2-Ig against Human ADA was performed. The affinity constant is 12.8 nM.

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Informations sur le produit

67870-1-PBS cible ADA dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADA Protéine recombinante Ag31326
• Nom complet: adenosine deaminase
• Masse moléculaire calculée: 363 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC040226
• Symbole du gène: ADA
• Identification du gène (NCBI): 100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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