Anticorps Monoclonal anti-ADAM10

ADAM10 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A6B7

N° de cat : 66620-1-PBS

Synonymes

ADAM 10, Disintegrin and metalloproteinase domain-containing protein 10, EC:3.4.24.81, KUZ, MADM

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Galerie de données de validation

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  WB analysis of THP-1 using 66620-1-Ig (same clone as 66620-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

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  WB analysis using 66620-1-Ig (same clone as 66620-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66620-1-Ig (same clone as 66620-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66620-1-Ig (same clone as 66620-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66620-1-PBS cible ADAM10 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADAM10 Protéine recombinante Ag23062
• Nom complet: ADAM metallopeptidase domain 10
• Masse moléculaire calculée: 748 aa, 84 kDa
• Poids moléculaire observé: 90-95 kDa
• Numéro d’acquisition GenBank: BC126253
• Symbole du gène: ADAM10
• Identification du gène (NCBI): 102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).

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