Anticorps Polyclonal de lapin anti-ADAM17

ADAM17 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 29948-1-AP

Synonymes

ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86, Snake venom-like protease

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Galerie de données de validation

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  WB analysis using 29948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29948-1-AP (ADAM17 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 29948-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 29948-1-AP (ADAM17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules MCF-7, cellules RAW 264.7, cellules SKOV-3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

29948-1-AP cible ADAM17 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAM17 Protéine recombinante Ag32418
• Nom complet: ADAM metallopeptidase domain 17
• Masse moléculaire calculée: 824 aa, 93 kDa
• Poids moléculaire observé: 90-110 kDa
• Numéro d’acquisition GenBank: BC136783
• Symbole du gène: ADAM17
• Identification du gène (NCBI): 6868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

Publications

Species	Application	Title
mouse	WB	Int J Oral Sci; Administration of Porphyromonas gingivalis in pregnant mice enhances glycolysis and histone lactylation/ADAM17 leading to cleft palate in offspring; Authors - Xige Zhao
human	IF	Mol Cell Endocrinol; Embryo-derived human chorionic gonadotropin promotes human decidualization through activating epithelial prostaglandin F2α secretion; Authors - Dan-Dan Jin
mouse	WB	Theranostics; Neutrophil-like pH-responsive pro-efferocytic nanoparticles improve neurological recovery by promoting erythrophagocytosis after intracerebral hemorrhage; Authors - Linfeng Fan
human	WB	Nat Commun; ADAM9 promotes type I interferon-mediated innate immunity during encephalomyocarditis virus infection; Authors - Lindsey E Bazzone
human	WB	Cancer Manag Res; DSG2 and c-MYC Interact to Regulate the Expression of ADAM17 and Promote the Development of Cervical Cancer; Authors - Li-Mian Song
mouse,human	WB	Arch Biochem Biophys; Maresin1 restrains chronic inflammation and Aβ production to ameliorate Alzheimer's disease via modulating ADAM10/17 and its associated neuroprotective signal pathways: A pilot study; Authors - Shuang Sun ; KD Validated

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