Anticorps Monoclonal anti-ADAM17

ADAM17 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2F4C2

N° de cat : 68725-1-Ig

Synonymes

ADAM 17, ADAM17, CD156b, cSVP, Snake venom like protease, TACE, TNF alpha convertase, TNF alpha converting enzyme

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Galerie de données de validation

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  WB analysis using 68725-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68725-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HeLa, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68725-1-Ig cible ADAM17 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADAM17 Protéine recombinante Ag34022
• Nom complet: ADAM metallopeptidase domain 17
• Masse moléculaire calculée: 824 aa, 93 kDa
• Poids moléculaire observé: 90-110 kDa
• Numéro d’acquisition GenBank: BC136783
• Symbole du gène: ADAM17
• Identification du gène (NCBI): 6868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

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