Anticorps Monoclonal anti-ADAM17

ADAM17 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F4C2

N° de cat : 68725-1-PBS

Synonymes

Snake venom-like protease, EC:3.4.24.86, Disintegrin and metalloproteinase domain-containing protein 17, cSVP, ADAM 17

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Galerie de données de validation

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  WB analysis using 68725-1-Ig (same clone as 68725-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68725-1-PBS in a different storage buffer formulation.

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  WB analysis using 68725-1-Ig (same clone as 68725-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68725-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50392-2

  Cytometric bead array standard curve of MP50392-2, ADAM17 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68725-2-PBS. Detection antibody: 68725-1-PBS. Standard:Ag34022. Range: 0.098-100 ng/mL.

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Informations sur le produit

68725-1-PBS cible ADAM17 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADAM17 Protéine recombinante Ag34022
• Nom complet: ADAM metallopeptidase domain 17
• Masse moléculaire calculée: 824 aa, 93 kDa
• Poids moléculaire observé: 90-110 kDa
• Numéro d’acquisition GenBank: BC136783
• Symbole du gène: ADAM17
• Identification du gène (NCBI): 6868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

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