Anticorps Monoclonal anti-ADAM8

ADAM8 Monoclonal Antibody for WB, FC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, FC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A12H2

N° de cat : 68113-1-PBS

Synonymes

MS2, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 8, Cell surface antigen MS2, CD156a

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Galerie de données de validation

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  WB analysis of human testis using 68113-1-Ig (same clone as 68113-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

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  WB analysis of HaCaT using 68113-1-Ig (same clone as 68113-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 68113-1-Ig (same clone as 68113-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68113-1-Ig (same clone as 68113-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 68113-1-Ig (same clone as 68113-1-PBS)

  1x10^6 A431 cells were surface stained with 0.8 ug ADAM8 Monoclonal antibody (68113-1-Ig, Clone:3A12H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were not fixed. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68113-1-PBS cible ADAM8 dans les applications de WB, FC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADAM8 Protéine recombinante Ag17262
• Nom complet: ADAM metallopeptidase domain 8
• Masse moléculaire calculée: 824 aa, 89 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC115404
• Symbole du gène: ADAM8
• Identification du gène (NCBI): 101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ADAM8 is a type I transmembrane (TM) glycoprotein consisting of an N-terminal prodomain, followed by a metalloproteinase-, disintegrin (DIS)-, cysteine-rich-, epidermal growth factor (EGF)-like- and TM domain and a cytoplasmic tail. Expression levels of ADAM8 in normal tissue are typically low and limited to a few distinct cell types in the lymphatic organs as components of the immune system, the central nervous system, bone, and the lung. This antibody can be used to detect endogenous ADAM8 with an apparent molecular weight of 90 kDa (the calculated MW ). ADAM8 also can be detectable as a band with a molecular weight (MW) of 98 kDa, slightly larger than the calculated MW (90 kDa) (PMID:11050116).

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