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Anticorps Monoclonal anti-ADAM8

ADAM8 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3A12H2

N° de cat : CL647-68113

Synonymes

CD156, CD156a, Cell surface antigen MS2, Disintegrin and metalloproteinase domain-containing protein 8, EC:3.4.24.-

Galerie de données de validation

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  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 647-conjugated ADAM8 Monoclonal ant (CL647-68113)

    FC experiment of A431 using CL647-68113

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 647 Adam8 Monoclonal Antibody (CL647-68113, Clone:3A12H2)(red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

Applications testées

Résultats positifs en FC (Intra)cellules A431,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-68113 cible ADAM8 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, porc

Réactivité Humain, porc
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène ADAM8 Protéine recombinante Ag17262
Nom complet ADAM metallopeptidase domain 8
Masse moléculaire calculée 824 aa, 89 kDa
Poids moléculaire observé90 kDa
Numéro d’acquisition GenBankBC115404
Symbole du gène ADAM8
Identification du gène (NCBI) 101
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADAM8 is a type I transmembrane (TM) glycoprotein consisting of an N-terminal prodomain, followed by a metalloproteinase-, disintegrin (DIS)-, cysteine-rich-, epidermal growth factor (EGF)-like- and TM domain and a cytoplasmic tail. Expression levels of ADAM8 in normal tissue are typically low and limited to a few distinct cell types in the lymphatic organs as components of the immune system, the central nervous system, bone, and the lung. This antibody can be used to detect endogenous ADAM8 with an apparent molecular weight of 90 kDa (the calculated MW ). ADAM8 also can be detectable as a band with a molecular weight (MW) of 98 kDa, slightly larger than the calculated MW (90 kDa) (PMID:11050116).

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