Anticorps Recombinant de lapin anti-ADAMTS2

ADAMTS2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241860B8

N° de cat : 84476-6-RR

Synonymes

ADAMTS-2, pNPI, PCPNI, PCINP, ADAMTS 2

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Galerie de données de validation

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  WB analysis using 84476-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84476-6-RR (ADAMTS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84476-6-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ADAMTS2 antibody (84476-6-RR, Clone: 241860B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84476-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84476-6-RR against Human ADAMTS2 were performed. The affinity constant is 0.472 nM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HT-1080, cellules PC-3
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84476-6-RR cible ADAMTS2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ADAMTS2 Protéine recombinante Ag34957
• Nom complet: ADAM metallopeptidase with thrombospondin type 1 motif, 2
• Masse moléculaire calculée: 135 kDa
• Poids moléculaire observé: 110~130 kDa
• Numéro d’acquisition GenBank: NM_014244
• Symbole du gène: ADAMTS2
• Identification du gène (NCBI): 9509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) is a metalloproteinase that plays a key role in the processing of fibrillar procollagen precursors into mature collagen molecules by excising the amino-propeptide. ADAMTS-2 can, like the procollagen C-proteinases, be regulated by transforming growth factor-β1 (TGF-β1), with implications for mechanisms whereby this growth factor effects net increases in formation of extracellular matrix. Mutations in the ADAMTS-2 gene cause dermatosparaxis in human (dermatosparactic type of Ehlers-Danlos syndrome, also known as Ehlers-Danlos syndrome type VIIC). Human ADAMTS-2 has eight potential sites for Asn-linked glycosylation and other mammals (PMID: 16046392, 12646579).

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