Anticorps Recombinant de lapin anti-ADAMTS4

ADAMTS4 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

4F9

N° de cat : 82744-2-RR

Synonymes

ADAM-TS 4, ADAMTS 4, ADAM TS4, ADAM TS 4, A disintegrin and metalloproteinase with thrombospondin motifs 4

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Galerie de données de validation

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  WB analysis using 82744-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82744-2-RR (ADAMTS4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  FC experiment of U2OS using 82744-2-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug ADAMTS4 Recombinant antibody (82744-2-RR, Clone:4F9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82744-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82744-2-RR against Human ADAMTS4 were performed. The affinity constant is 0.632 nM.

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules Caco-2, cellules HEK-293T, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82744-2-RR cible ADAMTS4 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ADAMTS4 Protéine recombinante Ag2443
• Nom complet: ADAM metallopeptidase with thrombospondin type 1 motif, 4
• Masse moléculaire calculée: 837 aa, 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC030812
• Symbole du gène: ADAMTS4
• Identification du gène (NCBI): 9507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).

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