Anticorps Polyclonal de lapin anti-ADAMTSL4

ADAMTSL4 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15304-1-AP

Synonymes

ADAMTS like 4, ADAMTS like protein 4, ADAMTSL 4, ADAMTSL4, TSRC1, UNQ2803/PRO34012

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Galerie de données de validation

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  WB analysis of mouse brain using 15304-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15304-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15304-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15304-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15304-1-AP

  IP Result of anti-ADAMTSL4 (IP:15304-1-AP, 4ug; Detection:15304-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of mouse skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu de muscle squelettique de souris, tissu hépatique humain, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu de cancer du côlon humain, tissu de muscle squelettique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15304-1-AP cible ADAMTSL4 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAMTSL4 Protéine recombinante Ag7781
• Nom complet: ADAMTS-like 4
• Masse moléculaire calculée: 117 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC027478
• Symbole du gène: ADAMTSL4
• Identification du gène (NCBI): 54507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IP,IF	Matrix Biol; Interactions between lysyl oxidases and ADAMTS proteins suggest a novel crosstalk between two extracellular matrix families.; Authors - Rohtem Aviram
human	IHC, WB	Front Mol Biosci; An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma.; Authors - Hongpan Zhang
human	WB	Oncotarget; Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.; Authors - Ya Tao
rat	IHC	Int J Nanomedicine; Pioglitazone-Loaded Cartilage-Targeted Nanomicelles (Pio@C-HA-DOs) for Osteoarthritis Treatment; Authors - Junyan Chen

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