Anticorps Polyclonal de lapin anti-ADARB1

• Phare
• Validé par KD/KO

ADARB1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 22248-1-AP

Synonymes

RED1, ADAR2, RNA-editing deaminase 1, EC:3.5.4.37, Double-stranded RNA-specific editase 1

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Galerie de données de validation

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  WB analysis using 22248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22248-1-AP (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22248-1-AP (ADARB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22248-1-AP

  IP result of anti-ADARB1 (IP:22248-1-AP, 4ug; Detection:22248-1-AP 1:1000) with HeLa cells lysate 1080 ug.

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  IHC staining of mouse brain using 22248-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22248-1-AP (ADARB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22248-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22248-1-AP (ADARB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules 4T1, cellules C2C12, cellules HeLa, cellules HepG2, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• RIP: See 2 publications below

Informations sur le produit

22248-1-AP cible ADARB1 dans les applications de WB, IHC, IF, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADARB1 Protéine recombinante Ag17743
• Nom complet: adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)
• Masse moléculaire calculée: 741 aa, 81 kDa
• Poids moléculaire observé: 74-86 kDa
• Numéro d’acquisition GenBank: BC065545
• Symbole du gène: ADARB1
• Identification du gène (NCBI): 104
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADARB1(Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).

Publications

Species	Application	Title
human	WB	Nature; Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.; Authors - Shangming Tang
human	WB,RIP	Basic Res Cardiol; Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAs.; Authors - Karoline E Kokot
mouse	IF	Sci Rep; Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.; Authors - Alessia Mirra
mouse	WB	FASEB J; Lung fibrosis is induced in ADAR2 overexpressing mice via HuR-induced CTGF signaling.; Authors - Ramani Soundararajan ; KO Validated
human	WB	J Cell Mol Med; Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis.; Authors - Lei Peng
human	WB	Front Pharmacol; Aberrant Expression of ADARB1 Facilitates Temozolomide Chemoresistance and Immune Infiltration in Glioblastoma.; Authors - Can Lu

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