Anticorps Polyclonal de lapin anti-ADAT2

ADAT2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 13621-1-AP

Synonymes

ADAT2, DEADC1, dJ20N2, TAD2

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Galerie de données de validation

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  WB analysis of A431 using 13621-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 13621-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 13621-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13621-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13621-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13621-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13621-1-AP

  IP result of anti-ADAT2 (IP:13621-1-AP, 4ug; Detection:13621-1-AP 1:300) with mouse brain tissue lysate 3000 ug.

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  IHC staining of human hepatocirrhosis using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13621-1-AP (ADAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13621-1-AP (ADAT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 13621-1-AP

  Immunofluorescent analysis of A431 cells using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, tissu cérébral de souris, tissu pancréatique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

13621-1-AP cible ADAT2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAT2 Protéine recombinante Ag4529
• Nom complet: adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae)
• Masse moléculaire calculée: 191 aa, 21 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC037955
• Symbole du gène: ADAT2
• Identification du gène (NCBI): 134637
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADAT2(tRNA-specific adenosine deaminase 2) is also named as DEADC1 and Belongs to the cytidine and deoxycytidylate deaminase family. It is a 24-kDa molecular mass protein that harbors all the conserved motifs required for deamination. ADAT2 and ADAT3 can function as a heterodimer which catalyses inosine formation at the wobble position (position 34) in eukaryotic tRNAs(PMID:12457566). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res; The structure of the mouse ADAT2/ADAT3 complex reveals the molecular basis for mammalian tRNA wobble adenosine-to-inosine deamination.; Authors - Elizabeth Ramos-Morales