Anticorps Polyclonal de lapin anti-ADCY6

ADCY6 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 14616-1-AP

Synonymes

EC:4.6.1.1, Ca(2+)-inhibitable adenylyl cyclase, ATP pyrophosphate-lyase 6, AC6

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Galerie de données de validation

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  WB analysis of human brain using 14616-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14616-1-AP (ADCY6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14616-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14616-1-AP (ADCY6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14616-1-AP

  IP result of anti-ADCY6 (IP:14616-1-AP, 4ug; Detection:14616-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below

Informations sur le produit

14616-1-AP cible ADCY6 dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADCY6 Protéine recombinante Ag6218
• Nom complet: adenylate cyclase 6
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: BC064923
• Symbole du gène: ADCY6
• Identification du gène (NCBI): 112
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	CoIP	Nat Commun; Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via Ca2; Authors - Wei-Yin Lin
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li
mouse	WB	J Cell Biol; Sonic Hedgehog activates prostaglandin signaling to stabilize primary cilium length; Authors - Shariq S Ansari
mouse	WB	Mol Metab; Purβ promotes hepatic glucose production by increasing Adcy6 transcription.; Authors - Linna Jia
human	WB	Mol Pharmacol; Activity of adenylyl cyclase type 6 is suppressed by direct binding of the cytoskeletal protein 4.1G.; Authors - Masaki Saito
human	WB	Pathol Oncol Res; Knockdown of GPSM1 Inhibits the Proliferation and Promotes the Apoptosis of B-Cell Acute Lymphoblastic Leukemia Cells by Suppressing the ADCY6-RAPGEF3-JNK Signaling Pathway.; Authors - Ye Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-27-2022)

1 and 2 : WT starved and ShhN stimulated. 3 to 6 are ADC6 KO cells with 2 different gRNAs. 3/4 is one gRNA and 5/6 is another one. 3 and 5 are starved while 4 and 6 are ShhN stimulated

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: NIH-3T3

ADCY6 Antibody Western Blot validation (1/500 dilution) in NIH-3T3 (Cat no:14616-1-AP)

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