Anticorps Polyclonal de lapin anti-Gamma Adducin

• Phare
• Validé par KD/KO

Gamma Adducin Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 17585-1-AP

Synonymes

ADD3, ADDL, adducin 3 (gamma), Adducin like protein 70, Gamma adducin

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 17585-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17585-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17585-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 17585-1-AP

  IP result of anti-Gamma Adducin (IP:17585-1-AP, 4ug; Detection:17585-1-AP 1:500) with NIH/3T3 cells lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules K-562, tissu rénal de souris
• Résultats positifs en IP: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

17585-1-AP cible Gamma Adducin dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Gamma Adducin Protéine recombinante Ag11318
• Nom complet: adducin 3 (gamma)
• Masse moléculaire calculée: 76 kDa, 79 kDa
• Poids moléculaire observé: 80 kDa, 90 kDa
• Numéro d’acquisition GenBank: BC062559
• Symbole du gène: Gamma Adducin
• Identification du gène (NCBI): 120
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Mol Cell Biol; QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer.; Authors - Jin-Zhu Wang
human	WB	Stem Cell Res Ther; Knockdown of long noncoding RNA SAN rejuvenates aged adipose-derived stem cells via miR-143-3p/ADD3 axis; Authors - Hewei Xiong ; KD Validated
human	WB	PLoS One; Dysferlin and other non-red cell proteins accumulate in the red cell membrane of diamond-blackfan anemia patients.; Authors - Esther N Pesciotta
human	WB	Cell Genom; Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders; Authors - Greta Pintacuda

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