Anticorps Monoclonal anti-ADH1B

• Phare
• Validé par KD/KO

ADH1B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

1F1B4

N° de cat : 66939-1-Ig

Synonymes

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125

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Galerie de données de validation

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  WB analysis using 66939-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66939-1-Ig

  WB result of ADH1B antibody (66939-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH1B transfected HepG2 cells.

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  WB analysis of pig liver using 66939-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 66939-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66939-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66939-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66939-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1B antibody (66939-1-Ig, Clone: 1F1B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HepG2, cellules HuH-7, cellules SMMC-7721, tissu hépatique de porc, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

66939-1-Ig cible ADH1B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADH1B Protéine recombinante Ag10630
• Nom complet: alcohol dehydrogenase 1B (class I), beta polypeptide
• Masse moléculaire calculée: 375 aa, 40 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC033009
• Symbole du gène: ADH1B
• Identification du gène (NCBI): 125
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

Publications

Species	Application	Title
human,mouse	WB,IHC	iScience; A positive feed-forward loop between Fusobacterium nucleatum and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis.; Authors - Chi-Yao Hsueh
mouse	WB	Front Pharmacol; Kinsenoside Alleviates Alcoholic Liver Injury by Reducing Oxidative Stress, Inhibiting Endoplasmic Reticulum Stress, and Regulating AMPK-Dependent Autophagy.; Authors - Limin Gao
human	WB	Front Genet; Analysis of characteristic genes and ceRNA regulation mechanism of endometriosis based on full transcriptional sequencing; Authors - Chengmao Xie
human	IHC	Cell Rep Methods; Modeling alcohol-associated liver disease in humans using adipose stromal or stem cell-derived organoids; Authors - Guoyun Bi
human	IF	Oncoimmunology; HIF2A mediates lineage transition to aggressive phenotype of cancer-associated fibroblasts in lung cancer brain metastasis; Authors - Muyuan You
human	IF	Cancer Commun (Lond); Nicotinamide N-methyltransferase negatively regulates metastasis-promoting property of cancer-associated fibroblasts in lung adenocarcinoma; Authors - Peiyu Wang

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