Anticorps Polyclonal de lapin anti-ADI1

ADI1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 15133-1-AP

Synonymes

Aci reductone dioxygenase, acireductone dioxygenase 1, ADI1, APL1, ARD, HMFT1638, MTCBP 1, MTCBP1, SIP L, SIPL

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Galerie de données de validation

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  WB analysis of human testis using 15133-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15133-1-AP (ADI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15133-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15133-1-AP (ADI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 15133-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 15133-1-AP (ADI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15133-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15133-1-AP (ADI1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 15133-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15133-1-AP (ADI1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, tissu de côlon de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

15133-1-AP cible ADI1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADI1 Protéine recombinante Ag7274
• Nom complet: acireductone dioxygenase 1
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC001467
• Symbole du gène: ADI1
• Identification du gène (NCBI): 55256
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADI1(acireductone dioxygenase 1 ) is also named as SIPL, ARD, APL1, HMFT1638, MTCBP-1, FLJ10913, MTCBP1 and belongs to the acireductone dioxygenase (ARD) family. It catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).The downregulation of ADI1 protein expression in human prostate cancer specimens argues that ADI1 potentially inhibits the growth that occurs during prostate cancer progression(PMID:17786183). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IHC	J Cell Biol; Pancreatic tumor cell metastasis is restricted by MT1-MMP binding protein MTCBP-1.; Authors - Li Qiang
human	WB	Oncotarget; Arginine deiminase expressed in vivo, driven by human telomerase reverse transcriptase promoter, displays high hepatoma targeting and oncolytic efficiency.; Authors - Hui Jiang
human	WB	Biochem Biophys Res Commun; Clusterin and Pycr1 alterations associate with strain and model differences in susceptibility to experimental pancreatitis.; Authors - Sapna Iyer

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