Anticorps Polyclonal de lapin anti-Adiponectin

Adiponectin Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 21613-1-AP

Synonymes

ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein

---

Galerie de données de validation

• 

  WB analysis of NIH/3T3 using 21613-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21613-1-AP (ADIPOQ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 21613-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 21613-1-AP (ADIPOQ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21613-1-AP (ADIPOQ antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brown adipose using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brown adipose using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21613-1-AP (ADIPOQ antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21613-1-AP (ADIPOQ antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 21613-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 21613-1-AP (ADIPOQ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules 3T3-L1
• Résultats positifs en IHC: tissu hépatique humain, tissu adipeux brun de souris, tissu cutané humain, tissu de cancer de la prostate humain, tissu de muscle squelettique de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IF: See 5 publications below

Informations sur le produit

21613-1-AP cible Adiponectin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Adiponectin Protéine recombinante Ag16304
• Nom complet: adiponectin, C1Q and collagen domain containing
• Masse moléculaire calculée: 244 aa, 26 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC096308
• Symbole du gène: ADIPOQ
• Identification du gène (NCBI): 9370
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Publications

Species	Application	Title
mouse	IF	Diabetes; PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ; Authors - Yi Zhong
human	WB	Int J Mol Sci; Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.; Authors - Weiyun Zhang
mouse	WB	Respir Res; Isthmin-1 attenuates allergic Asthma by stimulating adiponectin expression and alveolar macrophage efferocytosis in mice; Authors - Jong Huat Tee
human	WB, IF	Sci China Life Sci; Compression loading of osteoclasts attenuated microRNA-146a-5p expression, which promotes angiogenesis by targeting adiponectin.; Authors - Yue Wang
mouse	WB	Dig Dis Sci; Adiponectin Alleviates Intestinal Fibrosis by Enhancing AMP-Activated Protein Kinase Phosphorylation.; Authors - Minghao Xie
mouse	WB	BMC Nephrol; Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway.; Authors - Xiaohong Xu