Anticorps Monoclonal anti-Adiponectin

Adiponectin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(9)

CloneNo.

5D8A7

N° de cat : 66239-1-Ig

Synonymes

ADIPOQ, 30 kDa adipocyte complement-related protein, 5D8A7, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein

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Galerie de données de validation

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  WB analysis of human adipose using 66239-1-Ig

  human adipose tissue were subjected to SDS PAGE followed by western blot with 66239-1-Ig (ADIPOQ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brown adipose using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 66239-1-Ig (ADIPOQ antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 66239-1-Ig (ADIPOQ antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brown adipose using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human adipose-derived mesenchymal stem cells using 66239-1-Ig

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (orange), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, white), adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, green). Nuclei shown in red. ( Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using 66239-1-Ig

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, red), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, yellow). Nuclei shown in blue. (Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using 66239-1-Ig

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, red), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, yellow). Nuclei shown in blue. ( Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using 66239-1-Ig

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, white), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, red). Nuclei shown in blue. (Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of 3T3-L1 using 66239-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed 3T3-L1 cells using Adiponectin antibody (66239-1-Ig, Clone: 5D8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu adipeux humain
• Résultats positifs en IHC: rat brown adipose tissue, tissu adipeux brun de souris, tissu cutané de souris, tissu de cancer de la prostate humain, tissu de muscle squelettique de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules 3T3-L1, human adipose-derived mesenchymal stem cells

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

66239-1-Ig cible Adiponectin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Adiponectin Protéine recombinante Ag17383
• Nom complet: adiponectin, C1Q and collagen domain containing
• Masse moléculaire calculée: 244 aa, 26 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC096308
• Symbole du gène: Adiponectin
• Identification du gène (NCBI): 9370
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Publications

Species	Application	Title
mouse	WB	J Cell Physiol; Rapid responses of adipocytes to iron overload increase serum TG level by decreasing adiponectin.; Authors - Yuxiao Tang
mouse	WB	Food Funct; Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling.; Authors - Xiaoling Chen
pig	WB	Anim Biotechnol; Effect of dietary leucine supplementation on skeletal muscle fiber type transformation in weaning piglets; Authors - Xiaoling Chen
pig	WB	Anim Biotechnol; Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway.; Authors - Lu Xiang
human	WB,IF	Chem Biol Interact; Inhibitory effects of black phosphorus nanosheets on tumor cell proliferation through downregulation of ADIPOQ and downstream signaling pathways; Authors - Daxu Liu
mouse,human	WB	Invest Ophthalmol Vis Sci; Butyrate Ameliorates Graves' Orbitopathy Through Regulating Orbital Fibroblast Phenotypes and Gut Microbiota; Authors - Pingbo Ouyang

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