Anticorps Polyclonal de lapin anti-Adiponectin receptor

• Phare
• Validé par KD/KO

Adiponectin receptor Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(21)

N° de cat : 14361-1-AP

Synonymes

ADIPOR2, ACDCR2, adiponectin receptor 2, Adiponectin receptor protein 2, ADIPOR1

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Galerie de données de validation

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  WB analysis using 14361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14361-1-AP (Adiponectin receptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14361-1-AP (Adiponectin receptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14361-1-AP

  IP result of anti-Adiponectin receptor (IP:14361-1-AP, 4ug; Detection:14361-1-AP 1:500) with mouse liver tissue lysate 3040 ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules L02, tissu hépatique de rat, tissu placentaire humain
• Résultats positifs en IP: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

14361-1-AP cible Adiponectin receptor dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Adiponectin receptor Protéine recombinante Ag5744
• Nom complet: adiponectin receptor 2
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC051858
• Symbole du gène: Adiponectin receptor
• Identification du gène (NCBI): 79602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK, and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. The human ADIPOR2 gene maps to chromosome 12p13.31, and encodes a 386-amino acid protein with a molecular weight of 44 kDa. AdipoR2 may also exist as stable ~ 60 kDa dimers (PMID: 18842004). This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure; Authors - Rui Wang
mouse	IF	Redox Biol; Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction.; Authors - Yuan Liu
hamster	WB	Cancer Sci; Exosomal zinc transporter ZIP4 promotes cancer growth and is a novel diagnostic biomarker for pancreatic cancer.; Authors - Haoyi Jin
mouse	WB	Food Funct; Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling.; Authors - Xiaoling Chen
mouse	WB	Food Funct; D-chiro-Inositol facilitates adiponectin biosynthesis and activates the AMPKα/PPARs pathway to inhibit high-fat diet-induced obesity and liver lipid deposition.; Authors - Qiong Yang
rat	WB,IF	J Alzheimers Dis; Advanced Glycosylation End Products Induced Synaptic Deficits and Cognitive Decline Through ROS-JNK-p53/miR-34c/SYT1 Axis in Diabetic Encephalopathy; Authors - Rui Zhang

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